plasmid dna padvantage (Promega)
90
Structured Review
Promega
plasmid dna padvantage
Plasmid Dna Padvantage, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/padvantage%E2%84%A2+dna/padvantage+plasmid/10__1039_slash_d4pm00219a-51-5-27
Average 90 stars, based on 1 article reviews
Plasmid Dna Padvantage, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/padvantage%E2%84%A2+dna/padvantage+plasmid/10__1039_slash_d4pm00219a-51-5-27
Average 90 stars, based on 1 article reviews
plasmid dna padvantage - by Bioz Stars,
2026-09
90/100 stars
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Incubation:Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: The antibody proteins were purified from the transfected cell supernatants on proteinA columns and then buffer exchanged into 10 mM sodium succinate, 140 mM NaCl, pH 6.0, and concentrated using a Centricon-10 (Amicon). Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: On the following day, double-stranded DNA prepared as a calcium phosphate precipitate was added, followed by pAdVAntageTM DNA ( Cell Culture:Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: The antibody proteins were purified from the transfected cell supernatants on proteinA columns and then buffer exchanged into 10 mM sodium succinate, 140 mM NaCl, pH 6.0, and concentrated using a Centricon-10 (Amicon). Article Title: Anti-FcRH5 antibodies and immunoconjugates and methods of use Article Snippet: On the following day, double-stranded DNA prepared as a calcium phosphate precipitate was added, followed by pAdVAntageTM DNA ( |
![Effect of M protein on transcriptional activity of genes dependent on RNAPI. (A) BHK cells were cotransfected with pHrMr <t>plasmid</t> <t>DNA</t> and 0, 36, or 360 ng of in vitro-transcribed wt M mRNA. Cells that received no M mRNA were cotransfected with 360 ng of yeast RNA as a negative control. At 24 h posttransfection, nuclei were isolated and RNA transcripts were elongated in the presence of [α-32P]UTP. Labeled RNAs were isolated and hybridized to linearized pHrMr plasmid DNA fixed on nitrocellulose membrane filters. (B) BHK cells were cotransfected with pHrMr plasmid DNA and 0 or 360 ng of tsO82 M mRNA. Transcription of pHrMr DNA was assayed by nuclear runoff analysis as described above for panel A. (C) BHK cells were transfected with pHrMr DNA or no plasmid DNA as a control for the specificity of hybridization. Transcription of pHrMr DNA was assayed by nuclear runoff analysis as described above for panel A. (D) BHK cells were infected at a multiplicity of infection of 20 PFU/cell with wt or tsO82 virus. Mock-infected cells were used as a control. Nuclei were isolated 6 h postinfection, and RNA transcripts were elongated in the presence of [α-32P]UTP. Labeled RNAs were isolated and hybridized to a cDNA fragment of 18S rRNA immobilized on nitrocellulose membranes. (E) The data from four (A and D) or three (B) separate experiments were quantitated by densitometry and expressed as a percentage of the control without M mRNA for the transfection experiments and as a percentage of the uninfected control for the virus-infected cells. The data are means ± standard deviations.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0232/pmc00110232/pmc00110232__jv1080539002.jpg)
![Effect of M protein on transcriptional activity of genes dependent on RNAPII. (A) BHK cells were cotransfected with pSV2.CAT plasmid DNA and 0, 36, or 360 ng of in vitro-transcribed wt M mRNA. Cells that received no M mRNA were cotransfected with 360 ng of yeast RNA as a negative control. At 24 h posttransfection, nuclei were isolated and RNA transcripts were elongated in the presence of [α-32P]UTP. Labeled RNAs were isolated and hybridized to linearized pSV2.CAT plasmid DNA fixed on nitrocellulose membrane filters. (B) BHK cells were cotransfected with pSV2.CAT plasmid DNA and 0 or 360 ng of tsO82 M mRNA. Transcription of pSV2.CAT DNA was assayed by nuclear runoff analysis as described above for panel A. (C) BHK cells were transfected with pSV2.CAT DNA or no plasmid DNA as a control for the specificity of hybridization. Transcription of pSV2.CAT DNA was assayed by nuclear runoff analysis as described above for panel A. (D) BHK cells were infected at a multiplicity of infection of 20 PFU/cell with wt or tsO82 virus. Mock-infected cells were used as a control. Nuclei were isolated 6 h postinfection, and RNA transcripts were elongated in the presence of [α-32P]UTP. Labeled RNAs were isolated and hybridized to a cDNA fragment of α-tubulin mRNA immobilized on nitrocellulose membranes. (E) The data from four (A and B) or two (D) separate experiments were quantitated by densitometry and expressed as a percentage of the control without M mRNA for the transfection experiments and as a percentage of the uninfected control in the case of the virus-infected cells. The data are means ± standard deviations.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0232/pmc00110232/pmc00110232__jv1080539003.jpg)
![Effect of M protein on transcriptional activity of adenovirus VA genes dependent on RNAPIII. (A) BHK cells were cotransfected with pAdVantage (pAdV) plasmid DNA and 0, 36 or 360 ng of in vitro-transcribed wt M mRNA. Cells that received no M mRNA were cotransfected with 360 ng of yeast RNA as a negative control. At 24 h posttransfection, nuclei were isolated and RNA transcripts were elongated in the presence of [α-32P]UTP. Labeled RNAs were isolated and hybridized to linearized pAdV plasmid DNA fixed on nitrocellulose membrane filters. (B) BHK cells were cotransfected with pAdV plasmid DNA and 0 or 360 ng of tsO82 M mRNA. Transcription of pAdV DNA was assayed by nuclear runoff analysis as described above for panel A. (C) BHK cells were transfected with pAdV DNA or no plasmid DNA as a control for the specificity of hybridization. Transcription of pAdVantage DNA was assayed by nuclear runoff analysis as described above for panel A. (D) The data from four (A) or three (B) separate experiments were quantitated by densitometry and expressed as a percentage of the control without M mRNA. The data are means ± standard deviations.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0232/pmc00110232/pmc00110232__jv1080539004.jpg)